Amblyomma argentinae Neumann, 1905 (Acari: Ixodidae) is a hard tick that parasitizes mainly reptiles. During amphibians and reptiles samplings in Miraflores locality, Chaco Province, Argentina, we collected a total of 34 nymphs and one adult of A. argentinae from the colubrid snakes Chironius maculoventris, Philodryas erlandi, P. psammophidea, and the turtle Acanthochelys pallidipectoris. Additionally, we add other records of A. argentinae in lizards previously reported in the Semi-arid Chaco of Argentina, and provide information on the behavior of the snake P. erlandi infested with 19 nymphs. These findings represent the first records of parasite-host association for Argentina.